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plasmid pcdna3 myr ha akt1  (Addgene inc)
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Addgene inc plasmid pcdna3 myr ha akt1
(A) RBP2 is overexpressed in human NSCLC cell lines SK-MES-1, A549, SPCA-1 and H1975 compared to the human bronchial epithelial cell line BEAS2B cells. (B) Effects of RBP2 siRNA1, RBP2 siRNA2 and <t>pcDNA3-HA-RBP2</t> on the expression of the RBP2 protein.
Plasmid Pcdna3 Myr Ha Akt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pcdna3 myr ha akt1 - by Bioz Stars, 2026-03
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(A) RBP2 is overexpressed in human NSCLC cell lines SK-MES-1, A549, SPCA-1 and H1975 compared to the human bronchial epithelial cell line BEAS2B cells. (B) Effects of RBP2 siRNA1, RBP2 siRNA2 and pcDNA3-HA-RBP2 on the expression of the RBP2 protein.

Journal: PLoS ONE

Article Title: Retinoblastoma Binding Protein 2 (RBP2) Promotes HIF-1α–VEGF-Induced Angiogenesis of Non-Small Cell Lung Cancer via the Akt Pathway

doi: 10.1371/journal.pone.0106032

Figure Lengend Snippet: (A) RBP2 is overexpressed in human NSCLC cell lines SK-MES-1, A549, SPCA-1 and H1975 compared to the human bronchial epithelial cell line BEAS2B cells. (B) Effects of RBP2 siRNA1, RBP2 siRNA2 and pcDNA3-HA-RBP2 on the expression of the RBP2 protein.

Article Snippet: The plasmid pcDNA3 Myr HA Akt1 was purchased from Addgene (Plasmid 9008) .

Techniques: Expressing

(A) Silencing RBP2 expression in H1975 cells significantly decreased the phosphorylation of Akt, and the forced expression of RBP2 in SK-MES-1 cells increased the activity of Akt. (B) When Akt was constitutively activated in RBP2-siRNA2 H1975, the expression of HIF-1α and VEGF were increased compared to the control. The PI3K/Akt inhibitor LY294002 significantly inhibited the expression of HIF-1α and VEGF in pcDNA3-HA-RBP2 SK-MES-1 cells. (C) Westernblots showing the time course of Akt phosphorylation in RBP2-siRNA2 H1975 cells due to VEGF-165 (25 ng/mL). (D) In the presence of recombinant human VEGF-165 stimulation, the activation of Akt was increased in RBP2-siRNA2 H1975 cells and RBP2-overexpressing SK-MES-1 cells (25 ng/mL, 30 minutes).

Journal: PLoS ONE

Article Title: Retinoblastoma Binding Protein 2 (RBP2) Promotes HIF-1α–VEGF-Induced Angiogenesis of Non-Small Cell Lung Cancer via the Akt Pathway

doi: 10.1371/journal.pone.0106032

Figure Lengend Snippet: (A) Silencing RBP2 expression in H1975 cells significantly decreased the phosphorylation of Akt, and the forced expression of RBP2 in SK-MES-1 cells increased the activity of Akt. (B) When Akt was constitutively activated in RBP2-siRNA2 H1975, the expression of HIF-1α and VEGF were increased compared to the control. The PI3K/Akt inhibitor LY294002 significantly inhibited the expression of HIF-1α and VEGF in pcDNA3-HA-RBP2 SK-MES-1 cells. (C) Westernblots showing the time course of Akt phosphorylation in RBP2-siRNA2 H1975 cells due to VEGF-165 (25 ng/mL). (D) In the presence of recombinant human VEGF-165 stimulation, the activation of Akt was increased in RBP2-siRNA2 H1975 cells and RBP2-overexpressing SK-MES-1 cells (25 ng/mL, 30 minutes).

Article Snippet: The plasmid pcDNA3 Myr HA Akt1 was purchased from Addgene (Plasmid 9008) .

Techniques: Expressing, Phospho-proteomics, Activity Assay, Control, Recombinant, Activation Assay